PreBI is a server for predicting biological homo protein-protein interfaces in crystal protein structures, based on the strength of the protein-protein interactions.


Please input your E-mail address, your name and job title.
Upload coordinate file with PDB format of the crystal structure
including the annotation with regard to the symmetry operations
(the CRYST1 record including the unit cell parameters and the space group symbol is essential) or input the PDB ID. In addition, input one protein chain ID which indicates the chain identifier of protomer which is searched for the biological interface.

E-mail address is essential since we will return a mail with a URL at which you will find the results.

E-mail Address
Your Name
Job Title



Choose uploading the PDB formatted file or inputting PDB ID,
and input one specified protein chain ID.
If the protein of the query has no chain ID, please input '0' as the specified chain ID.

If all boxes are filled, the server will use the uploaded coordinates for the prediction.
Upload Coordinate File
Input PDB ID
specified CHAIN ID

Examples 1b67 (chain A), 1ci4 (chain A), 1f1m (chain A)
1qsd (chain A), 1rop (chain A), 3eip (chain A)



In default condition, the symmetry-related protomers in the unit cell and the adjacent 6 cells will be considered, because of the shortening of the computation time. If the user wishes the complete evaluation using the unit cell and the surrounding 26 cells, please check the box.
Calculate using the unit cell and the surrounding 26 cells







Reference
Yuko Tsuchiya, Kengo Kinoshita, Nobutoshi Ito, Haruki Nakamura
"PreBI: Prediction of biological interfaces of proteins in crystals"
Nucleic Acids Res. accepted.


Yuko Tsuchiya, Kengo Kinoshita, Nobutoshi Ito & Haruki Nakamura
(Institute for Protein Research, OSAKA UNIV., JAPAN)

Mail to pre-s_admin

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last updated 2010.03.11